Rational design of heat stable lyophilized rotavirus vaccine formulations

Rational design of stable lyophilized protein formulations: theory and practice.

Optimizing rational vaccine design

Amir Aharoni, Department of Life Sciences and the National Institute for Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, Israel. The size of the microbial world is beyond our imagination. In last year’s crystal ball article, Tom Curtis compared the size of the microbial world to the size of the universe pointing out that the number of microorganisms in the world is billion...

A heat-stable hepatitis B vaccine formulation.

The purpose of the present study was to develop a formulation of recombinant hepatitis B vaccine with improved stability at elevated temperatures. A validated in vitro antigen reactivity assay was used to measure the stability of the vaccine. The formulation development focused on modification of the interactions between the antigen and aluminum hydroxide adjuvant and subsequent optimization of...

The Rational Design of an AIDS Vaccine

The extraordinary genetic diversity and immune evasion of human immunodeficiency virus (HIV) pose significant challenges for vaccine development. AIDS vaccine design requires a scientifically driven, rational approach that encompasses the latest advances in viral molecular genetics, structural biology, and immunology.

Heat stability of the lyophilized Sabin poliovaccine.

In this study we lyophilized three types of live attenuated polioviruses (Sabin vaccine strains) and evaluated the lyophilized vaccine viruses' heat stability. The virus titers were measured after heating at 37 and 45 and then compared with the titers of conventional liquid vaccine viruses similarly treated. The results showed that lyophilization, while slightly reducing vaccine virus titers,...